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. 2022 Mar 1;8:21. doi: 10.1038/s41421-021-00363-1

Fig. 7. Naringenin attenuated the progression of AAA via inhibition of the NLRP3 inflammasome in a lysosome-dependent manner.

Fig. 7

a–f Western blot analysis of the protein levels of caspase-1 and IL-1β in cell lysates (a) and ELISAs of the supernatant (b) from the vehicle- or naringenin (200 µM, 12 h)-stimulated primary peritoneal macrophages followed by LPS (1 ng/ml, 3 h) and AngII (1 µM, 30 min) treatment. The data were analyzed using two-way ANOVA followed by Bonferroni test for post hoc comparison and are presented as the means ± SEM of six independent experiments. *P < 0.05. Western blot analysis and quantification of the protein level of caspase-1 in cell lysates (c) and ELISAs of the supernatant level of IL-1β (d) from the Ad-GFP (5 MOI) or Ad-TFEB (5 MOI)-infected primary peritoneal macrophages followed by LPS (1 ng/ml, 3 h) and AngII (1 µM, 30 mis) treatment. The data were analyzed using two-way ANOVA followed by Bonferroni test for post hoc comparison and are presented as the means ± SEM of six independent experiments. *P < 0.05. Western blot analysis and quantification of the protein level of caspase-1 in the cell lysates (e) and ELISAs of the supernatant level IL-1β (f) from vehicle- or naringenin (200 µM, 12 h)-stimulated primary peritoneal macrophages followed by LPS (1 ng/ml, 3 h) and AngII (1 µM, 30 min) treatment in the presence or absence of CQ (1 µM). The data were analyzed using two-way ANOVA followed by Bonferroni test for post hoc comparison and are presented as the means ± SEM of six independent experiments. *P < 0.05. g ELISAs of the plasma IL-1β level in the 4-month-old male ApoE−/− mice gavaged with water or naringenin (50 mg/kg/day) and infused with AngII (1000 ng/kg/min, 4 weeks). n = 6–8 for each group. The data were analyzed using two-way ANOVA followed by Bonferroni test for post hoc comparison and are presented as the means ± SEM. h Western blot analysis of the protein levels of caspase-1 in cell lysates from the vehicle- or naringenin (200 µM, 12 h)-stimulated primary peritoneal macrophages isolated from 8-week-old male TFEBflox/flox mice and TFEBMφKO mice followed by LPS (1 ng/ml, 3 h) and AngII (1 µM, 30 min) treatment. The data were analyzed using two-way ANOVA followed by Bonferroni test for post hoc comparison and are presented as the means ± SEM of six independent experiments. *P < 0.05. i ELISAs of the plasma IL-1β level in 12-week-old male TFEBflox/flox mice and TFEBMφKO mice underwent sham operation or were periadventitially treated with CaPO4 followed by vehicle or naringenin (50 mg/kg/day) gavage for 7 days. n = 6–14 for each group. The data were analyzed using two-way ANOVA followed by Bonferroni test for post hoc comparison and are presented as the means ± SEM, *P < 0.05.