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. 2022 Feb 28;5:175. doi: 10.1038/s42003-022-03104-6

Fig. 5. Intrarenal angiotensin II activates the SPAK–NCC pathway.

Fig. 5

a Representative immunoblotting (above) and quantitative analysis (below) of renal phosphorylated SPAK and total SPAK in the active phase in the four groups (n = 6 mice per group). Statistical analysis was performed using two-way ANOVA. b Serum aldosterone levels among the four groups (NSD, HSD, L-NAME + HSD: n = 5, l-NAME: n = 4). Statistical analysis was performed using the Tukey–Kramer method. c Representative immunoblotting (above) and quantitative analysis (below) of renal AGT (angiotensinogen) in the active phase among the four groups. Each point is presented as the mean ± SEM. *p < 0.05 (n = 6 mice per group). Statistical analysis was performed using two-way ANOVA. d Schematic summary illustrating the regulation of sodium and urine via the angiotensin II-SPAK–NCC pathway in the kidney of our novel nocturnal polyuria mouse mode. All data are expressed as the mean ± SEM, *p < 0.05.