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. Author manuscript; available in PMC: 2023 Mar 1.
Published in final edited form as: J Thromb Haemost. 2021 Dec 17;20(3):574–588. doi: 10.1111/jth.15616

Figure 4. Immunization of FVIII−/− mice with BDD FVIII or FVIII variants demonstrate similar antibody responses.

Figure 4.

Schematic of the immunization regimen by retro-orbital injection in 8-12 week old FVIII−/− mice with the BDD FVIII proteins (A). Anti-FVIII IgG antibody titers by ELISA (B) and inhibitory antibodies by Bethesda assay (C) in FVIII−/− mice are shown. Mice injected with normal saline (NS) served as a negative control. Differences in the median ELISA and Bethesda titers of each of the FVIII variants were compared to median titers of BDD FVIII mice by the non-parametric Mann Whitney U test. **P <0.01.