Table 1.
Summary of FVIII MAbs
| Anti-FVIII MAb | FVIII Domain | IgG Subclass | MAb Binding Epitope [17–20, 56, 59] | Inhibitory Titer (BU/mg IgG) | VWF Binding IC50 (μg/ml) | PL Binding IC50 (μg/ml) |
|---|---|---|---|---|---|---|
| 2-116 | A1 | IgG2aκ | E11-D15, E53-A78 | <1 | >10 | >10 |
| 2-76 | A2 | IgG2aκ | R484-I508 | 38,000 | >10 | >10 |
| 4A4 | A2 | IgG2aκ | D403-H444 | 40,000 | >10 | >10 |
| 4F4 | A2 | IgG2aκ | Indeterminate | 330 | >10 | >10 |
| 2-54 | A2 | IgG1κ | E604-R740 | 34,000 | >10 | >10 |
| 1D4 | A2 | IgG2aκ | E604-R740 | 7,000 | >10 | >10 |
| 2-113 | A3 | IgG1κ | K1818-Y1916 | 156 | >10 | >10 |
| 2A9 | C1 | IgG2aκ | S2063-I2071, N2129-K2136 | 23 | 1.1 | 0.9 |
| B136 | C1 | IgG2aκ | A2077-I2084 | 700 | 0.4 | 0.04 |
| I54 | C2 | IgG2bκ | E2181, D2187, S2206, K2207, H2211, L2212, & Q2213 | 1,300 | 0.02 | 0.02 |
| 1B5 | C2 | IgG2aκ | F2196, T2197, N2198, F2200, T2202, R2220, Q2222, N2225, E2228, K2239, L2252, S2254, H2315, & Q2316 | 930 | 0.05 | 0.03 |
| 2-117 | C2 | IgG2aκ | H2269, Q2270, T2272, L2273, V2282, R2307, & H2309 | >0.4 | >10 | >10 |
BU, Bethesda units; IC50, 50% inhibitory concentration; PL, phospholipid; VWF, von Willebrand factor.