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The proposed pathway from hydantoin to orotate. 1H NMR (in D2O) of a reaction aliquot from 90 mM hydantoin with 1.5 eq. of glyoxylic acid in pH 8.2 1.0 M NaHCO3 buffer stirred at 60 °C for 1 hour (A), for 24 hours (B), and for 96 hours (C).
