Figure 3.

pH dependence of pyruvate (black) and orotate (shaded) production from 90 mM hydantoin with 1.5 eq. of glyoxylic acid, heated at 80 °C for 96 h in phosphate buffer (pH 4–7, 11–12), bicarbonate buffer (pH 8–10), or 2.0 M KOH (pH 14).