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A) Synthesis of pyruvate in 1.0 M NaHCO3, pH 8.2, 60 °C for 96 h. B) Pyruvate produced from i) (5‐13C)hydantoin and ii) (4‐13C)hydantoin in 1.0 M NaHCO3 pH 8.2, with 1.5 eq. of glyoxylic acid heated at 60 °C for 72 h. C−H couplings are consistent with expected values for i. two‐bond and ii. three‐bond proton‐carbon couplings.
