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Standardization of phytic acid (PA) hydrolysis conditions. Optimization of the phytase concentration in PA hydrolysis (A). PA was treated with phytase in a series of concentrations for 30 min at 55°C. Optimization of the reaction time for PA hydrolysis (B). PA was treated with 0.75 mg/ml phytase at 55°C. PA standard curve (C). All values are means over two replications.
