Skip to main content
. 2022 Feb 15;16:823060. doi: 10.3389/fnins.2022.823060

FIGURE 1.

FIGURE 1

Mecp2 KO astrocytes in the motor cortex (layer I) exhibit cytoskeletal atrophy in early-symptomatic (P20) as well as in moderate (P40) and severe symptomatic animals (P70). (A) Representative images of brain coronal sections, with the motor cortex (layer I) highlighted in red. (B) Micrographs are representative images of WT and Mecp2 KO astrocytes immunostained for GFAP (green) and DAPI (blue) in the motor cortex (layer I) at P20. Scale bar = 10 μm. (C,D) The graphs show the total length of processes (C) and their number (D) in astrocytes in the motor cortex of WT and Mecp2 KO mice at P20, P40, and P70. Data are represented as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001 by Student’s t-test or Mann–Whitney test in accordance with data distribution. One-way ANOVA indicates no significant alteration in WT and KO astrocytes along time. (E–G) The graphs depict data from Sholl analysis, reporting the number of intersections of astrocyte processes with concentric circles, in the motor cortex of WT and Mecp2 KO mice at P20 (E), P40 (F), and P70 (G). Representative images of reconstructed astrocyte arbors by SNT plugin are reported above each graph. *p < 0.05, **p < 0.01, ***p < 0.001 by two-way ANOVA, followed by Sidak’s multiple comparison test. WT and Mecp2 KO astrocytes (P20: n = 36 WT and n = 35 KO; P40: n = 27 WT and n = 44 KO; P70: n = 50 WT and n = 53 KO; n indicates the number of cells) derived from at least three different animals per genotype (P20: N = 4 WT and N = 4 KO; P40: N = 3 WT and N = 5 KO; P70: N = 5 WT and N = 6 KO; N indicates the number of animals).