Heatmap of presence (red) and absence (white) of genes involved in the first line of Acidithiobacillia class acid resistance including spermidine biosynthesis; aquaporine (Aq) with an asparagine residue (Asn39); cell envelope; outer membrane proteins (Out. mem.); capsular polysaccharide biosynthesis with pathways Lpt-, Wzy-, and Kps-systems; potassium transporters; and hopanoid biosynthesis plus export. The color intensity represents higher gene copy number per genome. The presence of genes (black; right-hand side) denotes HGT events by genome context with mobile genetic elements, phylogenetic trees, or if it was signaled as part of an HGT event by HGTector (Zhu et al., 2014). T. tepidarius, Thermithiobacillus tepidarius DSM 3134; Am. Sulfuriphilus, Ambacidithiobacillus sulfuriphilus CJ-2; F. caldus, Fervidacidithiobacillus caldus ATCC 51756; A. marinus, Acidithiobacillus marinus SH; A. thiooxidans, A. thiooxidans ATCC 19377; A. ferrianus, A. ferrianus MG; A. ferriphilus-like, A. ferriphilus-like BY0502; A. ferrivorans, A. ferrivorans SS3; A. ferridurans, A. ferridurans JCM 18981; and A. ferrooxidans, A. ferrooxidans ATCC 23270 (shaded colors correspond to those used in Figure 1).