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. 2022 Feb 15;12:822229. doi: 10.3389/fmicb.2021.822229

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Copyright © 2022 González-Rosales, Vergara, Dopson, Valdés and Holmes.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Unrooted phylogenetic tree of concatenated amino acid sequences of speD and speE in the Acidithiobacillia and their best hits in the NCBI databases. Acidithiobacillaceae sequences group in a clade from neutrophilic T. tepidarius, and had highest similarity to homologs in extreme acidophiles of the Acidiferrobacter group. Acidophilic sequences are highlighted in red. Filled circles at the nodes indicate bootstrap support ≥60%. The scale bar represents 0.1 amino acid substitution per site.