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Itraconazole inhibits proliferation and induces G2/M cell cycle arrest of human cSCC cells. (A,B) A431 and Colo16 cells were treated with indicated concentrations of itraconazole for 36, 48 and 72 h. Inhibition of cell viability was analyzed by CCK8 assay. (C–E) Crystal violet-stained colonies under an inverted microscope. (F–H) Cell cycle analysis via flow cytometry. [mean ± S.D. (error bars), n = 3; *p ≤ .05; **p ≤ .01; ***p ≤ .001, compared with control group].
