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. 2022 Feb 15;13:828983. doi: 10.3389/fphar.2022.828983

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Copyright © 2022 Xu, Zhuo, Liu and Chen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Itraconazole affects human cSCC development by regulating HMGCS1 transcription. (A) The relative mRNAs expression levels of HMGCS1 were presented by qRT-PCR. (B) The relative proteins expression levels of HMGCS1 were presented by western blot. qRT-PCR analysis (C) and western blot (D) were performed to detect HMGCS1 expression in A431 cells that were transfected with sh-Con, sh-HMGCS1#2, sh-HMGCS1#3, and sh-HMGCS1#5. (E) A431 sh-Con and A431 sh-HMGCS1#2 cells were treated with indicated concentrations of itraconazole for 48 h. Inhibition of cell viability was analyzed by CCK8 assay. (F) The relative luciferase activity was detected by dual-luciferase reporter gene activity assay. [mean ± SD (error bars), n = 3; *p ≤ .05; **p ≤ .01; ***p ≤ .001, compared with control group].