Figure 3.

Identification of RMP51 by immunoblotting. RMP51 and crude microsomal proteins isolated from Al-treated root tips of wheat cv PT741 were separated by SDS-PAGE, immunoblotted, and probed with polyclonal antibodies specific to the V-ATPase B subunit (from mung bean) and the F₁F₀-ATPase α- and β-subunits (from S. cerevisiae). Antibody labeling was detected using horseradish peroxidase-conjugated secondary antibodies and chemiluminescent detection. Apparent molecular mass of the cross-reacting bands indicated on the right are estimates based on migration of prestained molecular mass markers.