FIGURE 6.

Increase in CB₂ and TNFα but decrease in GPER protein levels in the OVCAR-5 ectopic xenograft tumors of SCID-SHO female mice receiving chronic systemic administration of JWH-133. JWH-133 (1 mg/kg i.p.) chronic administration for 30 days starting on day 30 of tumor growth produced an increase in CB₂ (A, B) and TNFα (A, C) protein levels in the OVCAR-5 ectopic xenograft tumors of SCID-SHO female mice relative to the vehicle treated group. However, a decrease in GPER (A, C)) protein levels was in the OVCAR-5 ectopic xenograft tumors of SCID-SHO female mice receiving chronic administration of JWH-133 relative to vehicle group. Symbols represent the means ± SEM. (OVCAR-5 tumors in SCID-SHO female mice receiving vehicle, n = 3–6 SCID-SHO females; OVCAR-5 tumors in SCID-SHO female mice receiving JWH-133, n = 3–6 SCID-SHO females). *p < 0.033 unpaired t-test (t₄ = 2.50, one-tailed); ^p < 0.0058 unpaired t-test (t₇ = 3.387, one-tailed); +p < 0.0117 unpaired t-test (t₄ = 3.568, one-tailed).