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. 2022 Jan 30;12(3):638–648. doi: 10.1002/2211-5463.13369

Fig. 5.

Fig. 5

Effects of F. nucleatum on goblet cell metaplasia and MUC5AC expression in lungs of elastase‐treated mice. Elastase‐ and PBS‐treated mice were administered either F. nucleatum or the vehicle (Mock). The lungs were harvested at 1, 2, or 7 days after the administration of F. nucleatum. (A) AB/PAS‐stained bronchi from elastase‐ and PBS‐treated mice seven days after the administration of either F. nucleatum or vehicle (Mock). Scale bar = 100 µm. (B) Percentage of goblet cells seven days after the administration of F. nucleatum. The values are presented as the mean ± SEM, n = 7. *P < 0.05; ***P < 0.001. (C) Muc5ac mRNA expression in the lungs determined using real‐time RT‐PCR. The values are presented as the mean ± SEM, n = 7. **P < 0.01; ***P < 0.001. (D) Immunohistochemistry for MUC5AC on bronchi from elastase‐ and PBS‐treated mice 7 days after the administration of F. nucleatum or Mock. Scale bar = 100 µm. Statistical significance was evaluated using one‐way ANOVA with Tukey’s post hoc analysis (B and C).