Figure EV1. Production of Cxcl1 and Cxcl2 in neutrophils stimulated with bacterial nucleic acids.

• A–D
  Concentrations of Cxcl1 (A, and C) and Cxcl2 (B, and D) in supernatants of neutrophil cultures stimulated with increasing concentrations (10¹, 10², 10³, and 10⁴ ng/ml) of the indicated nucleic acids. mRNA, messenger RNA; rRNA, ribosomal RNA; sRNA small RNA.
• E, F
  Cxcl1 (E) and Cxcl2 (F) concentrations in supernatants of bone marrow‐derived neutrophils from mice with genetic deficiency in TLR7 or TLR13 after stimulation with rRNA or mRNA. Chemokine levels in supernatants were measured at 24 h after stimulation.

Data information: Data are expressed as means + SD of three independent experiments conducted in duplicate. *P < 0.05, **P < 0.01, ***P < 0.001 versus RNA (A and B), rRNA (C and D) or WT mice (E and F), determined by unpaired t‐test.