Figure EV4. ROS production in neutrophils stimulated with Fpr and TLR agonists.

• A, B
  ROS production in neutrophils stimulated with live GBS (MOI 200) or combinations of Fpr agonists (fMIFL, WKYMVM, or GBS formylated peptides, 50 µM) and TLR agonists (HK‐GBS, 10 μg/ml or bacterial RNA, 1 µg/ml). Phorbol‐12‐myristate‐13‐acetate (PMA, 25 ng/ml) was used as a positive control stimulus. Data from one representative experiment of three producing similar results. Cells were stained with the CellROX fluorescent reagent. The shadowed area indicates stimulated cells.
• C
  ROS production in neutrophils stimulated with increasing concentrations of fMIFL or WKYMVM (10, 20, and 50 µM), HK‐bacteria (GBS or E. coli, 10, 20, and 40 μg/ml) or increasing numbers of live bacteria (MOIs of 100, 200, and 400). PMA (25 ng/ml) was used as a positive control stimulus. Data are expressed as means + SD from three experiments conducted in duplicate. *P < 0.05, ***P < 0.001, as determined by unpaired t‐test.