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. 2022 Feb 3;41(5):e109386. doi: 10.15252/embj.2021109386

Figure 6. ROS production requires the synergistic activation of Fprs and TLRs.

Figure 6

  • A
    Effect of actinonin on Cxcl2 production by neutrophils. Neutrophils were stimulated with live GBS (MOI 5) in the presence or absence of the formyl peptidase inhibitor actinonin (32 µg/ml).
  • B, C
    ROS production in neutrophils from WT, MyD88, or Fpr1/2‐deficient mice stimulated with different doses of live bacteria.
  • D
    Effect of treatment with ROS scavengers/inhibitors on Cxcl2 production. Neutrophils were treated with the singlet oxygen scavenger MnTBAP (100 μM), the hydrogen peroxide scavenger DMTU (10 mM), the NADPH oxidase inhibitor DPI (20 μM) or the ASK1 inhibitor GS‐444217 (1 μM) for 1 h before the addition of live GBS (MOI of 5).

Data information: Data are means + SD from three independent experiments conducted in duplicate. *P < 0.05, **P < 0.01, ***P < 0.001, as determined by unpaired t‐test.