Figure EV1. Validation of PACE.

• A
  A scatter plot showing the reproducibility of the codon effects measured by PACE in two replicates.
• B
  A scatter plot comparing the codon effects measured by PACE in the absence (x‐axis) and in the presence of GFP MO (y‐axis).
• C
  A scatter plot comparing codon usage in the zebrafish genome (per thousand codons, x‐axis) and the codon effects measured by PACE (y‐axis).
• D
  A scatter plot comparing CSCs (Bazzini et al, 2016, x‐axis) and the codon effects measured by PACE (y‐axis).
• E, F
  Comparison of the codon effects measured by PACE (E) with the results of qRT–PCR (F). mRNA levels at 6 hpf relative to 2 hpf are shown. The experiments were repeated three times. Error bars show SD.

Data information: In (A–D), the regression line is shown in blue and the 95% confidence interval is shown in gray. r represents Pearson's correlation. The significance was calculated using Student’s t‐test.

Source data are available online for this figure.