Fig. 3.

Effect of GPR133 on cell‐cycle distribution in LUAD cells. (A) GSEA assays were performed to explore the mechanism of GPR133 function in LUAD cells. (B) GPR133‐coexpressed genes were analyzed by GO and KEGG using the clusterprofiler software package on the r platform. (C) In LUAD cells with accelerating GPR133 and in control groups, the expression levels of cell‐cycle biomarkers were evaluated by qRT‐PCR and western blot assays. Data were reported as mean ± SD for three independent experiments, and statistical analysis was performed via Student’s t‐test vs. Vec, **P < 0.01. (D) Flow cytometry assays were conducted to detect the cell cycle in these cells.