Figure 5. ABPs interfaces with actin.

1. Sequence alignment of three actins (Act_Sc, Act_N2, and Act_Ca; deep blue indicates conserved residues, light blue and white indicates nonconserved), indicating contacts between proteins used in the biomimetic assay (with Arp2/3 complex at the mother filament interface (M), with Arp2/3 complex at the daughter filaments interface (D), with tropomyosin (T), with WASP’s WH2 (W), with formin (F), with profilin (P), with ADF/cofilin (C), with capping protein (Z), at the protofilament interface (*) and laterally (^).
2. Schematic representation of actin 3D structure (1YAG; Vorobiev et al, 2003). Color dots correspond to positions where Act_Sc has different residues compared to Act_N2 (red) and Act_Ca (blue). Purple dots correspond to positions where both Act_N2 and Act_Ca have different residues compared to Act_Sc.

Data information: Abbreviations: Sc—S. cerevisiae, N2—Node 2, Ca—C. albicans (for more details, see Table EV1, Fig 1, and Appendix Fig S1B and C).