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. 2021 Dec 28;94(5):2416–2424. doi: 10.1021/acs.analchem.1c04108

Figure 4.

Figure 4

High-throughput screening of environmental microorganisms based on DPP activity. (a) Scheme overview of application of the dipeptidyl ACA substrate for high-throughput screening of DPP-producing bacteria. (b) and (c) Pseudocolor plot of FADS for high-throughput WODLs, sorted using (b) Met–Leu–ACA and (c) Leu–Asp–ACA. Blue and green correspond to areas of lower cell density, red and orange are areas of high cell density, and yellow is mid-range. (d) Abundance ratio of the top 10 OTUs of each sample. FADS (M–L–ACA activity) and FADS (L–D–ACA activity) refer to postsorting samples based on Met–Leu–ACA and Leu–Asp–ACA hydrolysis activity, respectively. The name in parentheses is the family to which each OTU belongs. The abundance ratio was calculated based on 16S rDNA sequence data performed with the MiSeq System (shown in the Supporting Information).