SOMCL-668 positively modulates the effect of PRE084 both in vitro and in vivo. (A) Representative traces of intrinsic excitability and spike frequency adaption. (B) Spike frequencies elicited at 140 pA and 360 pA, with or without bath application of PRE084. (C) Complete plot of spike frequency against step current (pA) with or without bath application of PRE084. (D) Representative traces of intrinsic excitability and spike frequency adaption for action potentials. (E) Spike frequencies elicited at 220 pA and 360 pA, with or without bath application of SOMCL-668. (F) Complete plot of spike frequency against step current (pA) with or without bath application of SOMCL-668. (G) Representative traces of intrinsic excitability and spike frequency adaption for action potentials. (H) Spike frequencies elicited at 180 pA and 360 pA, with or without bath application of PRE084 + SOMCL-668. (I) Complete plot of spike frequency against step current (pA) with or without bath application of PRE084 + SOMCL-668. (J) Experimental outline of behavioral study. Social interaction (K) is presented as interaction time (s) and novel object recognition is presented as exploration time (s) for the familiar and novel objects (L) and as discrimination index (M). Data are shown as mean ± SEM; n = 16–18 cells from 3–4 mice in electrophysiological tests and n = 10–12 mice in behavioral tests. Statistical analysis was by two-way ANOVA (C, F, I and L), one-way ANOVA (K and M) and Student’s paired t-test (B, E and H); * P < .05, ** P < .01, *** P < .001.