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. 2001 Apr;125(4):2104–2119. doi: 10.1104/pp.125.4.2104

Figure 5.

Figure 5

Neighbor-joining tree representing alignment of apyrases from selected sources. Sixteen partial sequences of plant apyrases and the yeast protein GDA1 were aligned for analysis. The apyrases fall into five distinct subfamilies. Mtapy1, Mtapy3, Mtapy4, a newly identified EST from M. truncatula, MtEST (GenBank accession no. AJ388942), LNP (accession no. AF139807), and GS52 (accession no. AF207688; Day et al., 2000) fall into the first class. Mtapy2 was found to fall into subfamily 2, along with an Arabidopsis apyrase (accession no. AF156783; the second Arabidopsis apyrase [accession no. AF141671] falls in a similar position, not shown), and an EST from cotton, GhEST (accession no. AI729322). Additional sequences include G. soja GS50 (accession no. AF207687; Day et al., 2000), D. biflorus Dbapyrase (accession no. AF156781), potato apyrase (accession no. U58597), tomato EST cLED13N21 (accession no. AI488720), rice OsEST, (accession no. AU065811), and the S. cerevisiae GDA1 (accession no. 6320793).