Table II.

CaCl₂-induced actin reorganization in guard cells

Time	Actin Pattern				No. of Cellsa	Stomatal Aperturec
	1	2	3	4
min	% of total					μm
0	45	44	11	0	480	12.8 ± 0.38 (60)
10	55	34	11	0	460	11.9 ± 0.38 (60)
30	29	21	37	13	480	3.4 ± 0.42 (60)
60	13	8	31	48	500	1.5 ± 0.36 (60)

Epidermal fragments were treated with 2 mm CaCl₂ prior to measurement of stomatal apertures and actin labeling. Actin organizations in guard cells observed in six epidermal fragments from three independent experiments were classified into four types as in Figure 1 and Table I.

^a No. of cells examined for changes in actin pattern. 

^b Average ± se (n).