Table 1. Binding Affinity Measured by Fluorescence Quenching and ELISA Assays.
system | GQD-based fluorescence quenching | ELISA-like assay |
---|---|---|
LL-37 & HNP1-attached GQDs | 8 ± 1 nM | 9 ± 1 nM |
LL-37-attached GQDs | 12 ± 1 nM | 13 ± 1 nM |
HNP1-attached GQDs | 120 ± 20 nM | 116 ± 20 nM |
LL-37 peptide | 16 ± 1 nM | |
HNP1 peptide | 160 ± 40 nM | |
ACE2 | 8 ± 1 nM | |
GQDs | 3.8 ± 0.6 μM | 3.2 ± 0.5 μM |