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. 2022 Feb 21;2022:2422790. doi: 10.1155/2022/2422790

Figure 5.

Figure 5

High dimensionality UMAP analysis of NK cells expressing cytotoxic and CD69 immune marker. (a) NDM, (b) PDM, and (c) DM expression and histogram profile or dot blot expression of NK cell expressing cytotoxic [PFN (red), GZE B (blue), GNLSN (pink)] and CD69 (green) marker clusters upon UNS, PPD, WCL, and mitogen antigen stimulation. (d) Reduced frequencies of NK cell expressing cytotoxic and CD69 immune marker in LTB comorbid individuals. The PBMCs were cultured using unstimulated, stimulated with Mtb antigens and mitogen antigens for 18 h, and the cytotoxic (PFN, GZE B, and GNLSN) and CD69 immune markers frequencies were determined. The unstimulated, PPD, WCL and mitogen antigen stimulated conditions in LTB DM (n = 20), LTB PDM (n = 20), and LTB NDM (n = 20) individuals are displayed. Each black, grey, and silver colour circle indicates each group, and geometric mean weas indicated using bars. The frequencies of net cytokine were determined by subtracting the UNS cytokine frequency from Mtb and mitogen antigen stimulated frequencies. The significant difference was measured using the Kruskal-Wallis test Dunn's multiple comparisons and they were mentioned using P values.