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. 2022 Feb 16;2022:1599747. doi: 10.1155/2022/1599747

Figure 2.

Figure 2

High serum levels of TMAO did not affect short-term brain injury after acute ICH. (a) Representative images of LFB/Cresyl Violet-stained brain sections on day 3 after sham operation or ICH, scale bar = 1 mm. A lack of staining indicates the lesion area; (A–D) The sham+control group, sham+choline group, ICH+control group, and ICH+choline group, respectively. (b) Brain lesion volume was measured with LFB/Cresyl Violet-stained brain sections. Quantification analysis revealed that brain lesion volume in mice fed with a choline diet was similar to that in mice fed with a regular diet on day three after ICH (n = 8 mice/group). (c) The water content in the ipsilateral striatum increased significantly regardless of regular diet or choline diet after ICH and TMAO did not affect the brain water content on day three after ICH; #p < 0.05 compared with the sham+control group; ∗p < 0.05 compared with the sham+choline group (n = 6 mice/group). (d) The brain swelling in mice fed with a choline diet is similar to that in mice fed with a regular diet after ICH (n = 8 mice/group).