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. 2022 Feb 10;2022:8622388. doi: 10.1155/2022/8622388

Figure 6.

Figure 6

Spinal c-Fos is activated by the increase in miRNA-22 during inflammatory pain. (a)-(b) Blocking miRNA-22 with its inhibitor (22-Ih) reduced the CFA-induced increase in c-Fos expression in the ipsilateral dorsal horn. ∗p <0.05, ∗∗p <0.01 versus the corresponding groups. n =5. Data were analyzed with a two-way ANOVA followed by post hoc Tukey test. (c)-(d) Inhibiting miRNA-22 by LV-22 reduced the CFA-induced increase in c-Fos expression in the ipsilateral dorsal horn. ∗p <0.05, ∗∗p <0.01 versus the corresponding groups. n =5. Data were analyzed with a two-way ANOVA followed by post hoc Tukey test. (e)-(h) Overexpression of miRNA-22 by its mimic (22-mics, (e)-(f)) or Lenti-22 ((g)-(h)) augmented the level of c-Fos expression in the dorsal horn of naïve mice. ∗∗p <0.01 versus the corresponding groups. n =5. Scale bar, 50 μm. Data were analyzed with a Student's t test.