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. 2022 Jan 1;33(1):ar10. doi: 10.1091/mbc.E21-05-0229

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FIGURE 2: — RS induces thick SFs. (A) Actin filaments (phalloidin, green) and nuclei (Hoechst, blue) in HFF-1 cells at different senescence levels of P2, P10, and P33. The bottom panel shows high magnification of the area outlined by the white box in the top image. (B, C) The number of individual SFs (B) and the ratio of the number of individual SFs with a thickness of ≤400 μm or of >400 μm to the whole population (C) at P2 (n = 20 cells, 291 SFs), P10 (n = 20 cells, 297 SFs), and P2 (n = 20 cells, 332 SFs) from N = 3 independent experiments. Scale, 100 μm.