Table 1.
Direct RNA sequencing alignment metrics (pass reads)
| Undiff1 | Undiff2 | Diff1 | Diff2TR1 | Diff2TR2 | Diff3 | Overall | |
|---|---|---|---|---|---|---|---|
| Number of reads | 468 790 | 778 037 | 518 176 | 806 067 | 867 371 | 634 226 | 4 072 667 |
| Median read length (nt) | 989 | 1009 | 922 | 994 | 1041 | 1042 | 1004 |
| Median read quality score | 9.8 | 10.2 | 9.9 | 10.0 | 10.0 | 9.9 | 10.0 |
| Longest alignment length (nt) | 13 439 | 15 081 | 12 600 | 13 739 | 13 624 | 14 362 | 15 081 |
| Reads aligned to genome (%) | 97.5 | 97.8 | 97.8 | 98.1 | 98.2 | 98.1 | 98.0 |
| Reads aligned to transcriptome (%) | 97.7 | 97.8 | 98.0 | 98.3 | 98.4 | 98.3 | 98.2 |