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. 2022 Feb 7;50(4):1815–1828. doi: 10.1093/nar/gkac052

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© The Author(s) 2022. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 4. — Dimerization capability of the RNA constructs. EMSA Native gels of the RNA constructs. (A) Monomer constructs (M) tt_P1c, tt_P1m, tt_p1n and tt_P1 form dimers (D) (lanes 4–6, 10). Constructs tt_A1 and ec_P1c do not dimerize (lanes 8–9). (B) Monomer constructs (M) tt_A1P1, tt_P2c and tt_P2 form dimers (D) (lanes 4–5,7). Construct tt_A2 does not dimerize (lane 6). (C) Monomer constructs (M) sa_p1c and ef_p1c form dimers (D), small amounts of higher order oligomers can also be seen at the upper part of the gel (lanes 4–5). (D) Monomer constructs (M) tt_A2P2 forms multimer (lanes 4). (A–D) Two DNA ladders and one RNA ladder were used as reference (lanes 1–3).