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. 2022 Feb 7;50(4):1815–1828. doi: 10.1093/nar/gkac052

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© The Author(s) 2022. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 5. — Determination of the K_d values of the RNA construct dimers. (A) The average mean fluorescence intensity of varying concentration of dimer vs. monomer obtained was plotted to determine the dissociation constant K_d. Each experiment was repeated three times. An error bar represents the standard deviation of mean. (B) A representative gel (of RNA construct tt_P1n) used to determine the K_d value. Increasing concentration of RNA solutions, from 0 μM (left) to 42 μM (right) were mixed with a constant concentration of labelled RNA (0.15 μM). Gels were imaged fluorescently.