Table 1.
RNA constructs used, their dimerization capability and activity in peptide bond formation
| RNA Construct | Organism origin | 2D PTC location | Length | Dimer Kd (μM) | Peptide bond | Remarks |
|---|---|---|---|---|---|---|
| tt_A1 | T. thermophilus |
|
64 | ✗ | ✗ | min-A |
| tt_P1 | T. thermophilus |
|
71 | ✓ 47.43 ± 7.10 | ✓ | min-P |
| tt_A1P1 | T. thermophilus |
|
135 | ✓ 8.55 ± 2.53 | ✓ | min-A + min-P |
| tt_A2 | T. thermophilus |
|
91 | ✗ | ✗ | A-reg (includes A loop) |
| tt_P2 | T. thermophilus |
|
102 | ✓ | ✗ | P-reg (includes P loop) |
| tt_A2P2 | T. thermophilus |
|
193 | ✓ | ✗ | A-reg + P-reg |
| tt_P2c | T. thermophilus |
|
98 | ✓ | ✗ | P-reg and GNRA tetraloop |
| tt_P1c | T. thermophilus |
|
67 | ✓ 10.57 ± 1.16 | ✓ | min-P + GNRA tetraloop |
| ec_P1c | E. coli |
|
67 | ✗ | ✗ | min-P + GNRA tetraloop |
| sa_P1c | S. aureus |
|
67 | ✓ 0.73 ± 0.19 | ✓ | min-P + GNRA tetraloop |
| ef_P1c | E. faecium |
|
67 | ✓ 1.00 ± 0.20 | ✓ | min-P + GNRA tetraloop |
| tt_P1m | T. thermophilus |
|
64 | ✓ 6.57 ± 1.28 | ✗ | min-P + UAA loop |
| tt_P1n | T. thermophilus |
|
65 | ✓ 32.11 ± 4.19 | ✗ | min-P + UUAA loop |