Figure 2.

Molecular analysis of transgenic cells. A, Amplification product of PCR using genomic DNA from different cell lines and mouse odc-specific primers: NT, non-transgenic cells; 2E, 2F, 8B, different transgenic lines; and P, control plasmid. B, Southern hybridization of PCR amplification products from Figure 2A above using DIG-labeled probe made from mouse odc-cDNA. C, Southern hybridization of HindIII-restricted genomic DNA from different cell lines using DIG-labeled probe made from mouse odc-cDNA. D, Slot-blot northern hybridization of total RNA from and NT and 2E cells using DIG-labeled probe made from mouse odc-cDNA.