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Percentage of positive Mycobacterium tuberculosis droplet digital PCR (ddPCR) assays by fluorescence amplitude intensity threshold. The shaded region is the fluorescence amplitude intensity cutoff that provided 100% discrimination between negative control samples and positive control samples (i.e., 4,500–5,500). Dashed Line A represents the optimal fluorescence amplitude intensity cutoff (5,000) selected for all subsequent M. tuberculosis droplet digital PCR analyses.
