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. 2001 Apr;125(4):2189–2202. doi: 10.1104/pp.125.4.2189

Figure 7.

Figure 7

Simultaneous application of MeJA and ethylene mimics the effect of M. sexta herbivory on the accumulation of NaPMT1 and NaPMT2 mRNA in roots and nicotine in shoots of N. attenuata plants. A, Individual hydroponically grown plants (n = 1) received the following leaf wounding treatments without (left, control) and with (right) 1-MCP pretreatment: leaf wounding (wound); leaf wounding plus M. sexta R (wound + R-treatment); M. sexta larval feeding for 40 to 80 min (M. sexta folivory). Harvests, leaf nicotine determinations, and NaPMT1- and NaPMT2-specific RNA blotting were performed as in Figure 5. A depicts one set of results from three replicate experiments. B, NaPMT1 mRNA (top, quantified by slot blot and phosphor imager) and nicotine (bottom) levels from plants treated as in A, are presented as percent of the initial (T = 0) level. B depicts the average values (±se) from three replicate experiments (n = 3).