Table 1.

Description of the diffusion tensor obtained by the model-free analysis of the ¹⁵N relaxation parameters. ¹⁵N spin relaxation data of non-modified and modified tRNA^fMet were analyzed with the program RELAX (67,68) resulting in the diffusion tensor and the model-free parameters (Supplementary Table S8-S12) of both RNAs. The parameters and the explanation of the diffusion tensor are given in the first two columns, the determined value at the given temperature in the following columns

		Unmodified tRNAfMet		Modified tRNAfMet
		25°C	40°C	25°C	40°C
Rotational correlation time	τm [ns]	17.4	9.05	20.4	13.0
Isotropic part of the diffusion tensor	D iso [106 rad/s]	9.56	18.4	8.19	12.8
Anisotropic part of the diffusion tensor	D a [106 rad/s]	5.94	11.2	−6.04	−8.22
Ratio between parallel to orthogonal part of the diffusion tensor	D ratio	1.78	1.76	0.41	0.47
Spherical angles describing the orientation of the main axis in the diffusion tensor	Theta [rad]	0.59	0.59	1.90	1.90
	Phi [rad]	1.57	1.57	2.95	2.95