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. 2022 Feb 8;50(4):2143–2156. doi: 10.1093/nar/gkac069

Figure 5.

Figure 5.

Replication fork progression and transcription dynamics in rpb1-1 cells. (A) Replication times for rpb1-1 CoD (blue) and HO cells (orange) measured under uninduced or induced conditions (uninduced, 98 cells for CoD and 110 cells for HO, induced, 100 cells for CoD and 97 cells for HO). (B) Normalized fluorescent intensity of the transcription site and arrays, as described in Figure 3B, averaged over 92 cells from a rpb1-1 HO strain. (C and D) Normalized fluorescent intensity of the transcription site and arrays for a subpopulation of 45 cells that showed faster than median replication times (C) and a subpopulation of 47 cells that showed slower than median replication times (D). See Table 1 for the distance between the phase transition midpoints and the GAL10 gene. See Supplementary Figure S9 for transcription levels within the labeled L1–L4 time windows and their statistical significance, see Supplementary Table S1 for the extent of each time window. All experiments were performed at 30°C.