. 2022 Feb 7;50(4):2128–2142. doi: 10.1093/nar/gkac058

Table 2.

Data collection parameters, image processing, map and model details, and model validation statistics

Data collection parameters
Microscope	Thermo Fisher Titan Krios	Image processing and map details
Detector	Gatan Bioquantum-K2	Micrographs (no.)	847
Detector mode	Counting	Initial particles (no.)	99 615
Voltage	300 kV	Final particles (no.)	59 889
Magnification	130 000 X (nominal)	Map and model resolutions
Electron dose	60 e^–/Å²	Consensus map	2.9 Å (FSC = 0.143)
Exposure time	8.0 s	50S map	2.8 Å (FSC = 0.143)
Dose per frame	1.20 e^–/Å²	30S body map	3.0 Å (FSC = 0.143)
Defocus range	–0.8 to –2.6 μm	30S head map	3.0 Å (FSC = 0.143)
Object sampling	1.07 Å/pixel	Model (fit to consensus map)	3.0 Å (FSC = 0.5)
Model details and validation statistics
Model composition		R.m.s. deviations ^a
Non-hydrogen atoms^a	138 824	Bond lengths (Å)	0.020
Protein residues^b	5925	Bond angles (°)	1.47
Nucleic acid residues^b	4329	Protein geometry validation ^a
Metal ions^a	3 Zn, 2 Mg	Rotamer outliers (%)	0.58
Ligand^a	2 ATP	Ramachandran outliers (%)	0.38
General validation ^a	Ramachandran favoured (%)	86.11
CC (model to map fit)^c	0.77	RNA geometry validation ^b
Clashscore	12.33	Sugar pucker outliers (%)	1.18
MolProbity score	2.24	Backbone conformation outliers (%)	29.96

^aObtained from Phenix refine log and phenix molprobity.

^bObtained from MolProbity web server.

^cCC = correlation coefficient, measure of fit into consensus map.