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. 2022 Feb 7;50(4):2005–2018. doi: 10.1093/nar/gkac054

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© The Author(s) 2022. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Prmt1 depletion accelerates XEN induction in mouse ESCs. (A) FACS was performed by labelling Gata6 and Nanog in mouse ESCs without or with XEN induction (upper panels). The percentage of Gata6+/Nanog– cells is shown in the lower panel. (B) FACS showed the impact of Prmt1 rescue in its KO cells. Based on the Prmt1 KO background, a Prmt1 rescue (Prmt1) and an enzyme-inactive (Prmt1m) cell lines were generated and used for XEN induction (upper panels). The percentage of Gata6+/Nanog– cells is shown in the lower panel. (C) The effect of Fur treatment on E14 cells. (D) FACS showed the impact of an inhibitor of Prmt1 (Fur) in E14 cells with and without XEN induction (upper panels). The percentage of Gata6+/Nanog- cells is shown in the lower panel. (E) IF assays showed the impact of Fur on the expression of Gata6 (green) and Nanog (red) in E14 cells with XEN induction. Nuclei were visualized with DAPI staining (blue). Yellow circles indicate Gata6+ cells.