Propidium iodide uptake into syn-safencin-treated E. coli. a Saline and b isopropyl alcohol (IP) were used as negative and positive controls, respectively. E. coli were treated with c 32 μM, d 16 μM, or e 8 μM syn-safencin. Images are representative of three biological replicates (n = 3). f FACs sorting analysis of syn-safencin-treated E. coli. Saline (brown) and IP (black) are used as negative and positive controls, respectively. Cells were treated with either 32 μM (red), 16 μM (blue), 8 μM (purple), 4 μM (green), or 2 μM (yellow) syn-safencin