Figure 7.

DNA-MPO complexes after stimulation with PMA in neutrophils incubated with G-CSF, LPS or TNF-α. Neutrophils were treated with G-CSF (A), LPS (B), TNF-α (C) or medium only for 6 h, 24 h, 48 h and 72 h. Extracellular MPO-DNA complexes were detected by sandwich ELISA: capture antibody was MPO-directed and detection of DNA by antibody for double-stranded DNA coupled with a peroxidase. Results were measured by fluorometry at 650 nm. Values represent means ± SD of n = 3. Differences between the untreated control group and one treated group each were calculated individually using student’s t-test. Levels of significance were set at < 0.05 (*), < 0.01 (**), < 0.001 (***).