Figure 5. Steady state and tumour challenge phenotypes of Foxp3^CreCcdc101^fl/fl mice.

(a) Quantification of IL-2⁺ or IFN-γ⁺ populations of splenic CD4⁺Foxp3⁻ and CD8⁺ T cells from indicated mice (~8 weeks old) (n = 4 mice each group). (b) H&E staining of indicated tissues from indicated mice (> 4 months old) (n = 5 mice each group). Arrows indicate various inflammatory features. (c–f) Indicated mice were inoculated with MC38 colon adenocarcinoma cells. (c) Tumour size and end point tumour weight (n ≥ 5 mice per group). (d) Intratumoural T_reg cells, non-T_reg immune cells, and myeloid cells were sorted at 19 d after tumour challenge, followed by scRNA-seq analysis (see Methods) (n = 2 replicates). UMAP embeddings of CD45⁺ immune cells grouped by genotype (left) and indicated immune cell subclusters (middle). Right, frequencies of indicated immune cell subclusters. (e, f) Quantification of the percentage of tumour-infiltrated CD8⁺ T (e) or T_reg cells (f, left), and Foxp3 mean fluorescence intensity (MFI; gated on CD4⁺Foxp3⁺ cells; f, right) at 19 d after tumour challenge (n ≥ 5 mice per group). Mean ± s.e.m. (a, c, e, f); **P < 0.01; ***P < 0.001; two-tailed unpaired Student’s t-test (a, c, lower; e, f); two-way ANOVA (c, upper). Data are representative of one (b, d–f) or two (a, c) experiments.