Fig 2. The effect of a hypothermic solution on trilineage differentiation of canine adipose-derived mesenchymal stem cells.

The stored cAD-MSCs were able to differentiate into adipocytes, chondrocytes, and osteocytes. The red-stained fat droplets in adipocytes indicate adipogenic differentiation potential. The blue color indicates that chondrocytes are capable of producing proteoglycan in the extracellular matrix. The osteocytes that had accumulated calcium are shown in red color. Scale bars = 300 μm.