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. 2022 Feb 17;11:e71864. doi: 10.7554/eLife.71864

Figure 3. Transcriptional heterogeneity within the VZ, SVZ and MZ in E12.5 GEs.

Figure 3.

(A) High Nestin- and Dcx-expressing cells (> 1.5 fold expression above normalized dataset) were extracted from the GE populations and replotted. UMAP plots are annotated by: expression of Nestin and Dcx (upper left), brain region (lower left), separated into VZ (high-Nestin, red), SVZ (Dcx-, Mki67-, and Ccnd2-expressing, light blue) and MZ (Dcx-positive and Ccnd2- and Mki67-negative, dark blue) (upper right), and putative cell clusters (lower right). (B) Heatmap of genes enriched in VZ, SVZ and MZ cells. Each column represents expression in a single cell, color-coded as per the color scale. (C) UMAP plots depicting the top five differentially expressed genes (DEGs) in the VZ, SVZ, and MZ regions. (D) Heatmap showing expression of top 5 DEGs in each cell cluster from (A), with colored bar depicting whether each cluster contains predominantly VZ (red), SVZ (light blue), or MZ (dark blue) cells.