| Strain, strain background (Mus musculus) |
Nes-dVenus |
RIKEN BioResource Research Center |
C57BL/6-Tg(Nes-d4YFP*)1HOkn,RRID:IMSR_RBRC04058
|
Pooled sexes |
| Strain, strain background (Mus musculus) |
Wild-Type |
The Jackson Laboratory |
C57BL/6 J000664,RRID:IMSR_JAX:000664
|
Pooled sexes |
| Sequence-based reagent |
Chromium Next GEM Single Cell 3' Kit |
10X Genomics |
v2 (120267) v3 (1000092) |
v2 & v3 |
| Commercial assay or kit |
RNAscope HiPlex Assays |
Advanced Cell Diagnostics |
324,102 |
v1 |
| Antibody |
Anti-GFP (Rabbit polyclonal) |
Invitrogen |
A11122,RRID:AB_221569
|
1:400 |
| Antibody |
Anti-Doublecortin (Chicken polyclonal) |
Abcam |
AB153668,RRID:AB_2728759
|
1:1,000 |
| Antibody |
Anti-Nestin (Rat Monoclonal) |
Millipore |
MAB353,RRID:AB_94911
|
1:100 |
| Software, algorithm |
Cell Ranger |
10X Genomics |
RRID:SCR_017344
|
v3.0.0 |
| Software, algorithm |
Seurat |
the Satija Lab at the New York Genome Center |
RRID:SCR_007322
|
v3.0.0 |
| Software, algorithm |
Slingshot |
Kelly Street and Sandrine Dudoit at UC Berkeley |
RRID:SCR_017012
|
https://github.com/kstreet13/slingshotv2.2.0 |
| Software, algorithm |
DoubletFinder |
Christopher S. McGinnis and Zev J. Gartner at UCSF |
RRID:SCR_018771
|
https://github.com/chris-mcginnis-ucsf/DoubletFinderv3.0 |
| Software, algorithm |
RNAscope HiPlexImage Registration |
Advanced Cell Diagnostics |
|
v1 and v2 |
| Software, algorithm |
Photoshop CC |
Adobe |
RRID:SCR_014199
|
20.0.9 |
| Software, algorithm |
ImageJ |
National Health Institution |
RRID:SCR_003070
|
http://imagej.nih.gov/ij/
|
