The distribution of Definiens percent positive by pathologist-assigned category. The overlap between between pathologist-assigned category and Definiens percent positive varied by marker, with some exhibiting substantial overlap (ER, AR, IR, H3K27, RXR, CD163 in stroma) and others exhibiting little or no overlap (Ki67, CD8, CD20, FASN, IGF1R, pmTOR, PTEN). ER, PR, AR, IR, H3K27, VDR, RXR, CD163-tumor, EPRS, FASN, IGF1R, pmTOR, PTEN: Left panel, negative; middle panel, low positive (1–10%); right panel, positive (>10%). CD163-stroma: Left panel, low positive (1–10%); right panel, positive (>10%). No cases were manually scored as negative. Ki67: Left panel, low proliferation (≤14%); right panel, high proliferation (>14%). CD20, CD8, CD4: Left panel, low (<10%); middle panel, moderate (10–50%); right panel, high (>50%).