Table 4.
Agreement between dichotomized Definiens percent positive and pathologist-assigned scores.
| Marker | Pathologist score | Definiens positive, N (%) | Definiens negative, N (%) | Percent agreement (%) | Cohen’s kappa (95% CI) |
|---|---|---|---|---|---|
|
| |||||
| ER | Low positive/Positive | 4106 (86.7) | 45 (15.7) | 86.5 | 0.36 (0.33–0.40) |
| Negative | 632 (13.3) | 242 (84.3) | |||
|
| |||||
| PR | Low positive/Positive | 2838 (79.5) | 63 (15.1) | 80.1 | 0.38 (0.35–0.41) |
| Negative | 732 (20.5) | 354 (84.9) | |||
|
| |||||
| AR | Low positive/Positive | 3168 (80.1) | 81 (43.8) | 79.1 | 0.13 (0.10–0.16) |
| Negative | 786 (19.9) | 104 (56.2) | |||
|
| |||||
| IR | Low positive/Positive | 82 (66.7) | 0 | 66.7 | N/A |
| Negative | 41 (33.3) | 0 | |||
|
| |||||
| H3K27 | Low positive/Positive | 119 (90.2) | 0 | 90.2 | N/A |
| Negative | 13 (9.9) | 0 | |||
|
| |||||
| VDR | Low positive/Positive | 108 (91.5) | 0 | 91.6 | 0.15 (−0.11–0.42) |
| Negative | 10 (8.5) | 1 (100) | |||
|
| |||||
| RXR | Low positive/Positive | 75 (83.3) | 0 | 83.3 | N/A |
| Negative | 15 (16.7) | 0 | |||
|
| |||||
| Ki67 | High proliferation | 4 (100) | 12 (48.0) | 58.6 | 0.23 (0.02–0.44) |
| Low proliferation | 0 | 13 (52.0) | |||
|
| |||||
| CD163, tumor | Low positive/Positive | 89 (76.7) | 0 | 76.7 | N/A |
| Negative | 27 (23.3) | 0 | |||
|
| |||||
| CD163, stroma | Low positive/Positive | 122 (100) | 0 | 100 | N/A |
| Negative | 0 | 0 | |||
|
| |||||
| CD20 | Moderate/High | 14 (93.3) | 8 (8.3) | 92.0 | 0.71 (0.54–0.89) |
| Low | 1 (6.7) | 89 (91.8) | |||
|
| |||||
| CD8 | Moderate/High | 34 (85.0) | 16 (20.5) | 81.4 | 0.61 (0.46–0.75) |
| Low | 6 (15.0) | 62 (79.5) | |||
|
| |||||
| CD4 | Moderate/High | 11 (61.1) | 31 (30.4) | 68.3 | 0.20 (0.03–0.36) |
| Low | 7 (38.9) | 71 (69.6) | |||
|
| |||||
| EPRS, tumor | Low positive/Positive | 69 (59.5) | 0 | 59.5 | N/A |
| Negative | 47 (40.5) | 0 | |||
|
| |||||
| EPRS, stroma | Low positive/Positive | 90 (92.8) | 12 (75.0) | 83.2 | 0.20 (−0.04–0.45) |
| Negative | 7 (7.2) | 4 (25.0) | |||
|
| |||||
| FASN | Low positive/Positive | 115 (97.5) | 0 | 97.5 | 0.39 (−0.15–0.93) |
| Negative | 3 (2.5) | 1 (100) | |||
|
| |||||
| IGF1R | Low positive/Positive | 84 (70.6) | 0 | 71.1 | 0.07 (−0.02–0.17) |
| Negative | 35 (29.4) | 2 (100) | |||
|
| |||||
| pmTOR | Low positive/Positive | 91 (87.5) | 0 | 89.0 | 0.62 (0.45–0.80) |
Percent agreement and kappa statistics were calculated by dichotomizing the Definiens percent positive, using the following cutpoints for positivity: ≥10% (CD20, CD8, and CD4); >14% (Ki67); or ≥1% (all other markers).